Maternal—Fetal rejection reactions are unconstrained in preeclamptic women
Fig 3
Regulatory T cells of preeclamptic women fail to suppress anti-fetal proliferative responses.
(A) Gating strategy for Treg identification. Gating strategy starting with Fig 1B lymphocyte gate and subjecting to S1A Fig Steps until identification of CD4+ T cells. (B) Proportion of total FoxP3+ Treg gated off the total CD4+ populations. (C) % of three Treg subtypes gated off the total CD4+ populations in the PB (left hand side) and UPI (right hand side). (D) Number of CD8+ T cells divided by Cytokine+ Activated Treg as determined in C. (E-G) Proliferative response of maternal PBL to stimulation with related- or unrelated lymphocytes. Donor PBL are exposed to lymphocytes from the indicated sources with (squares) or without (triangles) depletion of CD25+ cells. (E) Donor PBL of a healthy, non-pregnant woman are exposed to unrelated cord blood lymphocytes, (F) PBL of a healthy pregnant woman are exposed to cord blood lymphocytes from her baby and (G) PBL from a preeclamptic pregnant woman are exposed to cord blood lymphocytes from her baby. (H) Fold change in proliferation of healthy (circles) or preeclamptic (squares) pregnant women’s PBL exposed to cord blood lymphocytes of their babies after CD25-depletion. N = 8 normal pregnant women, N = 6 preeclamptic pregnant women total in two separate experiments, representative results are depicted. Statistical analysis in (B, C, D, H) used two-way Student’s T test, unpaired. (E-G) Statistical analysis used one-way ANOVA with Bonferroni post-test. In all test, *p<0.05, **p<0.01.