UVC radiation as an effective disinfectant method to inactivate human papillomaviruses
Fig 1
Differing efficacy profiles of disinfectants against HPV.
HPV16 (A) or HPV18 (B) virions were subjected to hard surface carrier tests based on the ASTM E1053-11 standard test method against the disinfectants indicated. Virus films were dried onto 50mm diameter ABS carriers in the presence of a 5% fetal bovine serum (soil) and 4ppm CaCO3 (hard water) before being disinfected according to the disinfectant or device manufacturer’s instructions. Viral films were assayed for infectivity using a quantitative RT-PCR based method detecting the spliced E1^E4 transcript in infected recipient cells. Post-disinfection infectivity was compared to input to determine log10 reductions. Each efficacy test was conducted in quadruplicate and was paired with a matched neutralization control. Data is expressed as an average of n = 3; error bars indicate standard deviation.