Collagen scaffold enhances the regenerative properties of mesenchymal stromal cells
Fig 5
Secretion of soluble factors by MSCs grown in plate or collagen scaffold.
MSCs were maintained in plates or collagen patches for 3 days before respective treatment and subsequent analysis. The basal secretion of cytokines/chemokines into culture medium (measured by ELISA) was similar by non-stimulated MSCs maintained in plates or in patches. The basal level of prostaglandin (PG) E2 was slightly, but significantly, higher in patch-MSC cultures. In plates, stimulation of MSCs with Poly (I:C) or LPS increased the level of IL6, IL8, IP10 and RANTES. In path-cultured MSCs, Poly (I:C) induced the secretion of IL6, IL8, IP10 or RANTES but the response to LPS was either absent or decreased. Stimulating MSCs with either Poly (I:C) or LPS had no effect on PGE2 or IL10 production by MSCs. In all experiments n = 3 MSC donors. Error bars are SEM. * represents the statistical difference between groups (*P <0.05; **P <0.01; ***P <0.001).