Production of proinflammatory mediators in activated microglia is synergistically regulated by Notch-1, glycogen synthase kinase (GSK-3β) and NF-κB/p65 signalling
Fig 2
LiCl decreases protein expression levels of NICD, Deltex, GSK-3β, MCP-1, TNF-α, and iNOS in a dose dependent manner in BV-2 cells challenged with LPS.
A shows specific bands of NICD (80kDa), Deltex (67kDa) and GSK-3β (46kDa). Bar graphs (B, C) show ratio of density changes by Image J; Western blotting (D) shows specific bands of MCP-1(12kDa), TNF-α (26kDa) and iNOS (130kDa). Note the decrease of these proteins by LiCl treatment. The values represent the mean ± SD in triplicate.