Intrapancreatic injection of human bone marrow-derived mesenchymal stem/stromal cells alleviates hyperglycemia and modulates the macrophage state in streptozotocin-induced type 1 diabetic mice
Fig 5
Intrapancreatic hBMMSC injection modulates the macrophage activation state.
Representative images of Iba1 and CD206 immunostaining in the islets (A) and exocrine pancreas (B) of STZ-untreated control mice (untreated) and STZ-treated mice on day 28 after intrapancreatic injection of hBMMSCs or the vehicle. Scale bars = 100 μm. Semi-quantitative analysis of islets (C and D) and the exocrine pancreas (E and F) for Iba1 (C and E) and CD206 (D and F) in untreated (n = 3), vehicle-treated (n = 6), and hBMMSC-treated (n = 6) mice. Data are expressed as the mean ± SEM (Tukey post-hoc test). (G) Iba1 immunoreactions (green) were merged with F4/80 (red) immunoreactions in the islets as indicated by insulin immunoreactions (pink) in vehicle-injected mice. (H) Iba1 immunoreactions (green) were merged with CD206 immunoreactions (red) in the adjacent area of the islets (insulin immunoreactions; pink) in hBMMSC-injected mice. (I) Iba1 immunoreactions (green) were merged with MHC class II (red) in the islets and adjacent in vehicle-injected mice. DAPI staining (blue). Scale bars = 25 μm.