Structural prediction of two novel human atypical SLC transporters, MFSD4A and MFSD9, and their neuroanatomical distribution in mice
Fig 1
Orthologue clustering and sequence topology.
Hidden Markov Models were utilised on proteomes from various species to identify orthologues to human MFSD4A and MFSD9. (A) Schematic representation of the branching order for orthologue proteins. Abbreviations: ac, anolis lizard; dr, zebrafish; ga, stickleback; gg, chicken; mm, mouse. Neither of the proteins was found in yeast, roundworm or fruit fly. MFSD4B was identified as orthologue to MFSD4A. Global pairwise alignments were run to calculate protein identities between human and animal orthologues, as listed in (B). The human protein sequence for MFSD4A (C) and MFSD9 (D) were depicted, where the 12 transmembrane segments (TMS) were underlined (TMS1-12). The first 6 TMS constitute the N-domain which is connected to the C-domain (TMS7-12) via a long cytoplasmic loop (grey). Both the N and C terminals were predicted to be localised on the cytoplasmic side of the membrane.