NIMA-related kinase 1 (NEK1) regulates meiosis I spindle assembly by altering the balance between α-Adducin and Myosin X
Fig 3
Lack of NEK1 results in abnormal meiosis I spindle morphology in spermatocytes and oocytes.
(a) Immunofluorescence (IF) against β-tubulin (green) and DNA staining with DAPI (magenta) showing a wildtype (top) and Nek1kat2j/kat2j spermatocytes (bottom), along with measurements of spindle length (b) and width (c). Black bars are means (μm) ± Standard deviation. (d) Immunofluorescence (IF) against β-tubulin (green) and DNA staining with DAPI (magenta) showing a wildtype (top) and Nek1kat2j/kat2j (bottom) oocytes, along with measurements of spindle length (b) and width (c). Black bars are means (μm) ± Standard deviation. * Indicates statistically significant differences (Unpaired t-test p < 0.05).