TLR4 as a negative regulator of keratinocyte proliferation
Fig 4
Growth of shTLR4 cells in nude mice.
(A) Knockdown of TLR4 induces growth of HaCaT cells in nude mice (injected 4X103 cells/mouse (mice, n = 6/group); p = 0.0002, t-test). The graphic represents the tumor growth (tumor volume) at different time intervals post injection. (B) Expression of TLR4 in the control and sh TLR4 tumors on end day. The differential TLR4 expression was evaluated on transcriptional level by qPCR using specific anti-human TLR4 primers. The tumor RNA and cDNA synthesis were performed using TRIzol reagent and RT respectively.