Biochemical and immunological characterization of a novel monoclonal antibody against mouse leukotriene B4 receptor 1
Fig 6
The 7A8 mAb depletes granulocytes and monocytes in vivo.
(A, B) WT mice were injected intraperitoneally with 7A8, 1A8, anti-Gr-1 mAbs, or PBS as a control. (A) PBL were collected at 1 day after mAb injection and stained with 7A8, anti-Gr-1, anti-F4/80, and anti-CCR2 mAbs. The staining was analyzed separately in Gr-1 and CD11b (upper panels), F4/80 and CD11b (middle panels), or CCR2 and CD11b (lower panels). (B) The percentages of Gr-1hiCD11bhi(CCR2-F4/80-) granulocytes (left panel), F4/80loCD11bmid(CCR2loGr-1mid) monocytes (middle panel), and CCR2hiCD11bhi(F4/80loGr-1hi) monocytes (right panel) within total PBL (n = 4–5) are shown. Cont: control. Data were analyzed by one-way ANOVA, followed by the Bonferroni post-hoc test. ns, not significant. *, p < 0.05; **, p < 0.01; ***, p < 0.001.