Biochemical and immunological characterization of a novel monoclonal antibody against mouse leukotriene B4 receptor 1
Fig 1
Establishment of a novel anti-mBLT1 mAb.
(A) The strategy for generation of anti-mBLT1 mAbs by immunization of BLT1-KO mice (BALB/c) with intact L1.2-mBLT1 cells. (B) Ab titers in the plasma of BLT1-WT and BLT1-KO mice after immunization with L1.2-mBLT1 cells (n = 6 for BLT1-WT mice, n = 8 for BLT1-KO mice). The mean fluorescent intensity (MFI) was determined by flow cytometry. The relative MFI was defined as the MFI of binding to CHO-mBLT1 cells divided by the MFI of binding to mock-transfected cells. Data were analyzed by two-way ANOVA. ***, p < 0.001. (C) SDS-PAGE analysis of 7A8 mAb purified from hybridoma supernatant using Protein G Sepharose.