Lateral flow nucleic acid biosensor for sensitive detection of microRNAs based on the dual amplification strategy of duplex-specific nuclease and hybridization chain reaction
Fig 5
(A) Photographs of detection results of strips with different miR-21 concentrations. (Left) Images were recorded with a scanner. (Right) Corresponding optical responses of red bands on the strip. Peak areas were analyzed with the Image J software. (B) Calibration curve of miR-21 sensing system. The curve was plotted as PT/PC vs logarithmic value of miR-21 concentration. Inset shows a linear relationship between PT/PC and the logarithm of miR-21 concentration. PT and PC are peak areas of test and control lines, respectively. Error bars are standard deviations from three independent measurements.