A novel class of chemicals that react with abasic sites in DNA and specifically kill B cell cancers
Fig 4
Covalent binding of AA3 to genomic DNA in vivo.
Fluorescence intensity due to Cy5 bound to genomic DNA is shown. (A) Comparison of labeling of HeLa and Daudi cell DNA with AA3. The cells were treated with AA3 for indicated lengths of time and this was followed by DNA extraction and reaction with Cy5 azide. (B) Comparison of labeling of DNA from untreated cells with cells pretreated with AA3. Genomic DNA was isolated, labeled with ARP and bound to Cy5-Streptavidin. (C) Comparison of binding of AA3 to genomic DNA in dead or dying cells with live cells. Cells were treated with AA3, dead cells were separated from live cells, the genomic DNA was extracted and reacted with Cy5 azide. The data are from six replicates, and the mean and standard deviation are shown. ** represents P-value <0.005, n.s. is not significant.