ERK1/2 signalling protects against apoptosis following endoplasmic reticulum stress but cannot provide long-term protection against BAX/BAK-independent cell death
Fig 8
Atg5-/- immortalised MEFs exhibit enhanced ER stress-induced cell death.
(A) Atg5 WT and Atg5-/- iMEFs were treated with the indicated concentration of Tg for 48 h. Cells were fixed and analysed by flow cytometry following propidium iodide staining. Results are the means ± S.D. of 3 independent experiments performed in technical triplicate. Statistics represent the results of two-way ANOVA followed by Bonferroni post-tests; **, p < 0.01; ***, p < 0.001. (B) Alternatively Atg5 WT and Atg5-/- iMEFs were treated as in (A) for 24 h. Whole cell lysates were fractionated by SDS-PAGE prior to analysis by immunoblotting using the indicated antibodies. Results shown are representative of 3 independent experiments.