TAT-mediated transduction of bacterial redox proteins generates a cytoprotective effect on neuronal cells
Fig 3
Transduction of TAT-LepFNR and TAT-LepHO into SH-SY5Y cells.
(A) Transduction time dependence. SH-SY5Y cells were incubated with 1 μM TAT-LepFNR (white diamonds) or TAT-LepHO (black circles) in serum free medium. Cell extracts were prepared at different time intervals and examined by Western blot analysis. As a control, cells were incubated for 1 h with 1 μM Lep-FNR/Lep-HO. (B) Transduction concentration dependence. SH-SY5Y cells were incubated for 1 h with TAT-LepFNR (white diamonds) or TAT-LepHO (black circles) in serum free medium at stated concentration of each protein. Cell extracts were prepared and examined by Western blot analysis. As a negative control, cells were incubated for 1 h with 3 μM Lep-FNR/Lep-HO. Band density was calculated relative to a loading control of βIII-tubulin. Images are representative of 3 different experiments.