Directional migration of mesenchymal stem cells under an SDF-1α gradient on a microfluidic device
Fig 5
Numbers of extravasating and directionally migrating MSCs in the direction of the SDF-1α gradient under different conditions.
(a) Control group; no inhibitor and no SDF-1α gradient. *p>0.05 (b) MSCs under SDF-1α gradient *p<0.05 (c) MSCs under AMD3100 (CXCR4 antagonist) treatment. *p>0.05 (d) MSCs under Y-27632 (Rho-ROCK inhibitor) treatment *p<0.05. (e) MSCs under NSC23766 (RAC inhibitor) treatment *p<0.05. (f-j) Graph of numbers of extravasated MSCs moving toward and away from the SDF-1α gradient. Y-axis represents numbers of cells, and X-axis represents number of days. White arrows indicate the extravasated MSCs. Paired t-test was applied for number of MSCs migrating on each side for each conditions with n = 8.