Clinical implications of carcinoembryonic antigen distribution in serum exosomal fraction—Measurement by ELISA
Fig 2
Effects of thawing temperature on serum exosomal CEA values and quantity.
A: Western blotting for CD63 of exosome from frozen serum samples from normal healthy subject that were thawed at 4, 25, and 37°C. B: Each color indicates the results obtained from exosome quantitation for frozen serum samples from the same patient that were thawed at 4, 25, and 37°C. C: Each color indicates exosomal CEA values measured using ELISA for frozen serum samples from the same patient that were thawed at 4, 25, and 37°C. The same colors in B and C correspond to the same patients. D: Exosomal CEA values for frozen serum samples from eight normal healthy subjects that were thawed at 4, 25, and 37°C.