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A new holistic 3D non-invasive analysis of cellular distribution and motility on fibroin-alginate microcarriers using light sheet fluorescent microscopy

Fig 5

Cell distribution analysis performed onto single LSFM imaging.

Representative images of MSCs loaded onto the fibroin-alginate microcarrier at day 3 (A) and 8 (B) of culturing after the initial loading. Superimposed green and red channels (Calcein-AM and Ethidium homodimer-1 respectively), single blue channel (Hoechst nuclear marker) and superimposed (merge) green, red and blue channels are shown.

Fig 5

doi: https://doi.org/10.1371/journal.pone.0183336.g005