Determination of the intracellular concentration of the export chaperone SecB in Escherichia coli
Fig 3
Determination of the cellular concentration of SecB.
The culture was grown in minimal medium. (A) Immunoblot of a standard curve (lanes 1–4) created by adjusting purified SecB to contain the same amount of total cellular content as that of the unknown sample by addition of a secB null cell culture: lanes 1–4 contain purified SecB loaded at 20, 40, 60, 80 ng mixed with cell culture to correspond to amount in lanes 5–8, lanes 5–8 contain the cell culture to be quantified for content of SecB loaded at 5, 10, 15, 20 μl. (B) Quantification of the intensity on the immunoblot for the standard, lanes 1–4 in (A). (C) Quantification of the intensity for the unknown, lanes 5–8 in (A).