Response surface methodology based extraction of Tribulus terrestris leads to an upsurge of antilithiatic potential by inhibition of calcium oxalate crystallization processes
Fig 8
Polarization micrographs: Morphology modulation and inhibition of oxalate crystals growth by statistically optimized aqueous extracts of T.terrestris on oxalate injured renal epithelial NRK-52 cells.
Polarization microscopic images indicating the morphology modulation efficacy and reduction in crystals adherence potency of AE1 and AE2 in oxalate injured NRK-52E cells. Cells treated with serum free media considered as untreated control and Cystone (50 μg/mL) were taken as positive control. Images were captured at 20X magnification; scale bar 100 μm. Multiple fields were assessed. Inset images showing the zoomed view of crystals for assessment of shape and morphology of crystals. a: Control, b: Injured with 2 mM oxalate, c,d and e: treated with AE1 at concentration (10 μg/mL), (25 μg/mL), (50 μg/mL) respectively, and f, g, h treated with AE2 at concentration (10 μg/mL), (25 μg/mL), (50 μg/mL), respectively, i: Cystone treated (50 μg/mL).