Comparative pathogenomics of Clostridium tetani
Fig 3
FGI is a mobile element in C. tetani.
Annotation and alignment of the FGI genomic region across sequenced C. tetani strains. The FGI contains at least 80 predicted proteins that include the flagellar structural and polysaccharide processing enzymes in the sialic-acid like biosynthetic pathway (legionaminic acid and pseudaminic acid). Aligned regions that encompass fla flagellin genes (shown in blue, CTC01691—CTC01715) span 30 kb between 1,801,310–1,829,794 (C. tetani E88 reference). Shaded regions define the boundaries of highly conserved sequences across strains, e.g. fla CTC01691 –predicted protein adjacent to CheY for strains ATCC 453/454, ATCC 9441, C2/E88, and GTC-14772. See shaded region that are conserved between GTC-14772 and 12124569/184.08 (predicted proteins 1–20). Clusters of orthologous genes and moderate conservation with known FGI from the genus Clostridium (C. botulinum, C. carboxyvidorans, C. lundense, C. sporogenes, and C. tunisiense) are labelled. Predicted Y1 transposases (green) not conserved in French strains 184.08 and 12124569, contain a predicted group IIc intron (element 22) and reverse transcriptase (element 23). Predicted glycosylation enzymes (red), transporters (magenta), CheY two-component system (yellow), and proteins of unknown function (black) are shown.