Anaplasma phagocytophilum infection modulates expression of megakaryocyte cell cycle genes through phosphatidylinositol-3-kinase signaling
Fig 2
A. phagocytophilum infection alters cell cycle gene expression in MEG-01 cells.
QRT-PCR analysis showing levels of CDC2 (A), CD25A (B), cyclin E (C), CDK5 (D), CDK8 (E) and cyclin G1 (F) in uninfected (UI) and A. phagocytophilum-infected (I) MEG-01 cells at days 1 and 7 p.i. The mRNA levels of cell cycle genes were normalized to human beta-actin mRNA levels. QRT-PCR was performed on samples generated from three independent experiments (3 samples/experiment) and analyzed in duplicates. The P value (P<0.05) calculated from Student’s t test was considered significant.