Distinct age and differentiation-state dependent metabolic profiles of oligodendrocytes under optimal and stress conditions
Fig 1
Morphology and survival of cell cultures under optimal and stress conditions.
Panels A-C: Adult brain derived OLs–illustration of expression of OL lineage markers O4(red)/Olig2(green) (Panel A) and O4(red)/MBP (green) (Panel B) under optimal conditions (N1) at day 1, and MBP (green) expression on day 12 (Panel C) post isolation. MBP immunoreactivity in early cultures (day 1 Panel B) is mainly punctate in cell bodies whereas immunoreactivity is observed in the cell processes that are extended during the culture period (panel C). Panels D-E: Post natal OLs differentiated in vitro (Panel D) at day 8 post-isolation express MBP; MBP+ cells ~ 70% and OPCs (Panel E) express O4 at day 4 post isolation under optimal conditions (N1); O4+ cells ~ 85%, All cultures were also stained with the nuclear marker, Hoechst. Scale Bar = 10μM. Panels F-H: Cell death under optimal (N1), LG and NG conditions were measured with TUNEL assay for adult OLs (Panel F, N1 vs. NG *p<0.05); post natal OLs differentiated in vitro (Panel G, N1 vs. LG *p<0.05, N1 vs. NG ***p<0.001 and LG vs. NG ***p<0.001); and post natal OPCs (Panel H, N1 vs. LG **p<0.01, N1 vs. NG ***p<0.001).