ACE phenotyping in human heart
Fig 5
Dependence of ACE activity on pH.
The assays of the activity of the purified heart and lung ACEs toward 0.5 mM Z-Phe-His-Leu (A) and 1.3 mM Hip-His-Leu (B) were performed in 25 mM acetate-MES-Tris-borate buffer, containing 0.15 M NaCl and 1 μM ZnCl2. Each value is a mean of several (2–3) experiments in duplicates.