Increased ParB level affects expression of stress response, adaptation and virulence operons and potentiates repression of promoters adjacent to the high affinity binding sites parS3 and parS4 in Pseudomonas aeruginosa
Fig 4
ParB excess induces the expression of chromosomal bexRp-lacZ transcriptional fusion.
PAO1161::bexRp-lacZ strain contains bexRp-lacZ transcriptional fusion inserted in the intergenic region of PAO1161 genome. White colony from L agar with X-gal was inoculated and used as a recipient in conjugation with either S17-1 (pKGB8) or S17-1 (pKGB9 araBADp-parB) donor cells. Conjugants were grown on selective L agar plates supplemented with X-gal. The photographs show representative plates of PAO1161::bexRp-lacZ with both plasmids.