Bortezomib initiates endoplasmic reticulum stress, elicits autophagy and death in Echinococcus granulosus larval stage
Fig 2
Expression of endoplasmic reticulum chaperones, UPR transducers and autophagic related genes in E. granulosus larval stages.
(A) Reverse transcription (RT)–PCR analysis of Eg-cnx, Eg-grp78, Eg-ire2 and Eg-xbp1 from total RNA of protoscoleces (PTS) incubated for 48 h under control conditions (Co) or treated with 5 μM Bz or 10 μM Rm. Amplification of Eg-actin I (actI) was used as a loading control. Molecular sizes of amplicons are indicated with arrowheads. (B) Quantitative PCR was carried out under the same conditions as indicated in A. Values are means ± S.D. of three independent experiments. Asterisks indicate significant differences. (C) RT–PCR analysis of, Eg-cnx and Eg-grp78 from total RNA of metacestodes (MTC) incubated for 48 h under control conditions (Co) or treated with 5 μM Bz or 10 μM Rm. (D) RT–PCR analysis and (E) quantitative PCR of Eg-atg genes from total RNA of protoscoleces (PTS) carried out under the same conditions as indicated in A (F). RT–PCR analysis of Eg-atg genes from total RNA of metacestodes (MTC) carried out under the same conditions as indicated in C.