Irisin regulates cardiac physiology in zebrafish
Fig 3
Irisin downregulated mRNA expression of PGC-1 alpha, myostatin-a and b and upregulated troponin C mRNA expression in zebrafish.
In vitro studies showed irisin (0.1 nM, 10 nM) downregulated relative mRNA expression of PGC- 1 alpha (A) in zebrafish heart (n = 8/ group). However, irisin (10 nM) upregulated troponin C relative mRNA expression in zebrafish skeletal muscle (B). Irisin (10nM) downregulated myostatin-a relative mRNA expression in zebrafish heart and skeletal muscle (B). Irisin (10 nM) downregulated the relative mRNA expression of myostatin-a in zebrafish heart and muscle (C). Irisin (0.1 nM, 10 nM) downregulated myostatin-b relative mRNA expression when compared to control in zebrafish heart (D). The mRNA expression data was normalized to 18s RNA. Asterisks denote significant difference between the tissues (* p<0.05). Data are presented as mean + SEM. One-way ANOVA (non-parametric) followed by Tukey’s multiple comparison test was used for statistical analysis.