The extracellular matrix and focal adhesion kinase signaling regulate cancer stem cell function in pancreatic ductal adenocarcinoma
Fig 5
The loss of FAK inhibits self-renewal.
(a) Frequency of ALDH+ MIA PaCa-2 and Capan-1 cells expressing scrambled control (shCtrl) or FAK shRNA (shFAK) following treatment with doxycycline for 96 hours. Data represent mean ± SD (n = 3) of shCtrl versus shFAK; *P < 0.05. (b) In vitro colony formation by MIA PaCa-2 and Capan-1 cells expressing scrambled control (shCtrl) or FAK shRNA (shFAK) following treatment with doxycycline for 96 hours on type I collagen. Data represents ± SD (n = 4) of control versus hairpin; **P < 0.001. (c) In vivo subcutaneous tumor growth by MIA PaCa-2 cells expressing scrambled control (shCtrl, n = 4) or FAK shRNA (shFAK, n = 7) following treatment with doxycycline. Error bar represents SD; *P = 0.02.