Easy labeling of proliferative phase and sporogonic phase of microsporidia Nosema bombycis in host cells
Fig 1
(A) Validation of pCold I-β-tubulin vector by PCR and Bam HI/Sal I enzyme digestion. ~1300 bp products were amplified by PCR or cleaved from recombinant vector. (B) SDS-PAGE of proteins expressed in Escherichia coli Rosetta. Recombinant β-Tubulin protein was induced to express at 37°C and 16°C. pCold I vector transformed E. coli Rosetta were induced for expression at 37°C as a control. (C) Immunoblot for β-Tubulin in total protein of Nosema bombycis mature spore. The antibody recognized a 50 kDa band which was consistent with prediction.