Lymphatic endothelial progenitors originate from plastic myeloid cells activated by toll-like receptor-4
Fig 8
NF-κB inhibitors significantly suppress MLT.
Four sets of human monocytes were differentiated with LPS, HMGB1 or nab-PXL as described under Methods. One set was treated with only TLR4 ligands and served as control (A). Three other sets were additionally treated with (B) 10 nM of isohelenin, (C) 5 μM of PDTC, or (D) 10 nM of leptomycin-B. After 4 days of treatment, mRNA was extracted and used to determine expression of the indicated lymphatic genes. The analyses for each condition and gene target were performed in triplicate. Results are presented as mean fold-changes of beta actin normalized values ± S.E.M. Note 30-fold difference in the scale of the Y-axis of control plotted data (A) and that from NF-κB inhibitors treated cells (B-D).