Fate of tenogenic differentiation potential of human bone marrow stromal cells by uniaxial stretching affected by stretch-activated calcium channel agonist gadolinium
Fig 6
Effect of gadolinium on strain-induced decrease in DNA counts.
The cells were fixed and DNA stained by Hoechst (blue). (A) The nuclei of the cells were visualized using confocal microscope and counted. (B) In the presence of Gd3+, 8% + 1 Hz for 72 h significantly decreased DNA count. The result was expressed as a mean ± 1 SD for five randomly selected fields in 3 independent experiments. Statistical significance (p < 0.05) was represented by asterisk which was compared to unstrained group with gadolinium treated. S-: no mechanical stimulation; S+: cyclic stretching applied; B+: with SACC inhibitor, gadolinium.