Enzymatic activity necessary to restore the lethality due to Escherichia coli RNase E deficiency is distributed among bacteria lacking RNase E homologues
Fig 5
Effects of the ribonucleolytic activity on the restoration of CFA in Δrne E. coli.
Cultures of MT1288 (a), MT1315 (b), MT1200 (c), MT1266 (d), or MT983 (e) were spread onto LB or M9 plates (0.6% gellan gum) with various carbon sources containing (0.1% ara) or lacking [ara(-)] 0.1% L-(+)-arabinose, as indicated. Appropriate concentrations of IPTG (10 μM for MT983; 50 μM for MT1315; no IPTG (leaky expression) for MT1200, MT1266, and MT1288) were added to the plates. Plates were scanned after incubation at 37°C for 6 days (6D) or for 14 days (14D), as indicated. Glyce glycerol, Pyru pyruvate.