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Nonenzymatic glycosylation of human serum albumin and its effect on antibodies profile in patients with diabetes mellitus

Fig 8

Direct binding ELISA of serum auto-antibodies in (a) T2DM (n = 50) (c) T1DM (n = 50) (e) GDM (n = 50) (g) T2DM+CKD (n = 50) with native and 400 mg/dL glucose modified HSA and normal human sera (NHS) served as control in (b) (n = 50), (d) (n = 50), (f) (n = 50) and (h) (n = 50).

The plate was coated with the respective antigens (10μg/mL). A p value <0.05 considered to be significant. ns = non-significant

Fig 8

doi: https://doi.org/10.1371/journal.pone.0176970.g008