Oxidative profiling of the failing right heart in rats with pulmonary hypertension
Fig 1
Hemodynamics characteristics of the OVA/Sugen5416 model of PAH.
(A) Schematics of the rat model of PAH induced by OVA and Sugen5416. Rats were injected intraperitoneally on Days 1 and 8 with 1 mg of OVA with Imject Alum Adjuvant (OVA/Alum) for sensitization. On Day 15, rats were subjected to 30 min of 1% OVA inhalation with a nebulizer, which continued twice a week for four weeks. Sugen5416 (20 mg/kg body weight) was injected subcutaneously (S.C.) once a week on the day after OVA inhalation. Hemodynamic measurements were performed, rats were sacrificed, and lung and heart tissues were obtained on Day 50. (B) Representative traces of hemodynamic measurements taken from the RV using a Millar catheter. (C) The bar graph represents means ± SEM of systolic RV pressure. * denotes a value that is significantly different from the control value at P < 0.05 (N = 6).