p38 MAPK activation and H3K4 trimethylation is decreased by lactate in vitro and high intensity resistance training in human skeletal muscle
Fig 3
La-induced delay in late differentiation in C2C12 cells is similar to p38 MAPK-inhibition in cells treated with 0 mM La DM + SB203580.
A Myogenin- (green, Alexa 488) and DAPI-staining (blue) in C2C12 myoblasts before (pre) and after 5 days of incubation with the respective DM. B Representative western blots for Pax7, Myf5, myogenin, and MHC. Actin was used as a loading control. C, E, G, I Ratio of positively stained nuclei in IF analysis. D, F, H, J Quantification of western blots for Pax7 (C, D), Myf5 (E, F), myogenin (G, H), and MHC (I, J). Data presented as mean ± SD. * indicates statistical significance, p < 0.05. For every marker analysed, differences between 0 mM La DM or 0 mM La DM + DMSO and 0 mM La DM + SB203580 or 20 mM La DM were significant (p<0.01).