Transcriptome of interstitial cells of Cajal reveals unique and selective gene signatures
Fig 6
Identification of multiple Ano1 transcriptional variants.
(A) A genomic map view of 10 Ano1 variants expressed in JICC and CICC. Five alternatively starting exons (E1, E2, E15, E17, and E24) are circled and three differentially spliced exons (E8, E15, and E17) are boxed. (B) Expression levels of total Ano1 mRNAs in JICC, CICC and tissues of origin. (C) Expression levels of Ano1 transcriptional variants in JICC, CICC and tissue of origin. (D) PCR validation of Ano1 exons with alternative transcriptional start sites (ATSS) and alternative spliced sites (ASS) in isolated ICC and their corresponding tissue. E8-ASS, E15-ASS, and E17-ASS contained 2 bands amplified from spliced variants. PCR products of E15-ASS were digested by Alu to distinguish E15 skipping transcripts (undigested) from E15 containing transcripts (digested). NTC is non-template control. Primer sets were designed from variant exons in the regions of interest (see S11 Table for primer sequences).