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Cloning retinoid and peroxisome proliferator-activated nuclear receptors of the Pacific oyster and in silico binding to environmental chemicals

Fig 4

Oyster embryo development after 21 h of exposure to TBTO, rosiglitazone (Rosi).

All-trans retinoic acid (ATRA) and perfluorooctanoic acid (PFOA). a–k) Example of oyster development under a light (grey) and differential interference contrast (blue) microscope. l) Percentage of perfect developed D-shaped larvae and abnormal developed larvae grouped in in four abnormal development categories. Bold numbers next to pie charts: percentage perfect D-shaped (left) and total abnormal D-shaped (right) larvae. Non-bold numbers: percentage of abnormal developed categories to total percentages of abnormal developed D-shaped larvae. The standard error of percentage larval development did not exceed ±6% (not shown). Italic numbers: swimming larvae in the water column per ml (la/ml). Oyster individual categories: perfectly developed D-shaped larvae (a, g; blue), extruding velum (b, h; red), protruding soft tissue, (c, I; green), shell partly developed (d; purple), arrested shell/shell not developed (e-f, k; orange).

Fig 4

doi: https://doi.org/10.1371/journal.pone.0176024.g004