Hypoxia-inducible factor-2α stabilizes the von Hippel-Lindau (VHL) disease suppressor, Myb-related protein 2
Fig 4
A central region in HIF-2α interacts with B-Myb.
(A) Schematic representation of the HIF-2α deletion mutants used in this study. (B) Co-immunoprecipitation between full-length B-Myb and deletion mutants of HIF-2α. Wild-type or deletion mutants of 3×FLAG-HIF-2α were coexpressed with full-length 3×HA-B-Myb in HEK293T cells, and cell lysates were subjected to immunoprecipitation (IP) with an anti-HA antibody and immunoblotted with anti-FLAG or anti-HA antibodies. Deletion mutants of HIF-2α containing a central region between residues 436 to 600, which fully spans the transactivation domain and partially spans the oxygen-dependent degradation domain, interact with full length B-Myb. Representative data from three independent experiments are shown.