Role of NeuroD1 on the negative regulation of Pomc expression by glucocorticoid
Fig 6
Effects of DEX on NeuroD1 promoter activity in AtT20 cells.
(A) Effect of DEX on full-length NeuroD1 promoter activity. AtT20 cells transiently transfected with 300 ng mNeuroD1-Luc (ND full: -2.2-kb/+150) and 150 ng pRSV-β-gal were treated with DEX (1 nM, 10 nM, or 100 nM) or 0.1% ethanol (vehicle control) for 24 hrs before the luciferase assay. Results are expressed as percentages of control (100%). Each point represents mean ± SEM (n = 4). **P<0.01 and ***P<0.001 vs control. (B) Effect of DEX on NeuroD1 promoter deletion mutants. AtT20 cells transiently transfected with 300 ng ND full, ND mut1, ND mut2, ND mut3, or ND mut4 and 150 ng pRSV-β-gal were incubated in the presence (100 nM) or absence of DEX for 24 hrs before the luciferase assay. Results are expressed as percentages of each control (100% in pGL3-Basic). Each point represents mean ± SEM (n = 4). ***P<0.001 vs control. NS denotes “Not Significant.”