Evaluation of circulating miRNAs during late pregnancy in the mare
Fig 2
Differential expression of circulating miRNAs in pregnant mares.
Expression of circulating miRNAs was analyzed using quantitative PCR to identify miRNAs with significantly different expression during pregnancy. Samples were grouped into pregnant (8, 9, 10 m gestation, postpartum (PP)) or non-pregnant (diestrus) samples and analyzed by one-way ANOVA corrected for false discovery rate (Benjamini-Hochberg; P < 0.05), with post-hoc analysis performed by student’s t-test (P < 0.1). Data were normalized by ΔCt = Ct(target)−Ct(mean of the sample), with each point representing the ΔCt for an individual mare. A lower ΔCt value indicates a higher expression level; for each 1-point reduction in ΔCt, an approximately 2-fold increase in expression can be assumed. Significantly different samples are indicated by varying superscripts.