ANKRD54 preferentially selects Bruton’s Tyrosine Kinase (BTK) from a Human Src-Homology 3 (SH3) domain library
Fig 2
Characterization of the high-affinity ANKRD54/BTK interaction.
(A) Transfection by electroporation of Namalwa cells and (B) transiently-transfected Cos7 cell lysate mixed with Namalwa (Nam) lysate, followed by co-immunoprecipitation (IP) of endogenous BTK. The missing expression of BTK from the whole cell lysate (WCL) of untransfected Cos7 cells (lane 1) shows that these cells do not express BTK.