Structural basis for non-genuine phenolic acceptor substrate specificity of Streptomyces roseochromogenes prenyltransferase CloQ from the ABBA/PT-barrel superfamily
Fig 4
SrCloQ prenylated 4’-hydroxy-7-methoxyflavone products.
RP-UHPLC-UV (A) and MS in PI (B) profiles of 4’-hydroxy-7-methoxyflavone and prenylated products (14, 15 and 16 in Table 1) after incubation of 4’-hydroxy-7-methoxyflavone with DMAPP and SrCloQ. MS2 spectra of C-prenylated isomer (m/z 359, Na adduct) at retention time 17.1 min (15, panel C) and O-prenylated isomer at 18.1 min (16, panel D). The proposed RDA fragmentation pathway is shown as inset.