Proteolysis of mature HIV-1 p6 Gag protein by the insulin-degrading enzyme (IDE) regulates virus replication in an Env-dependent manner
Fig 5
Inhibitors of the metalloprotease IDE block the in vitro degradation of sp6.
(A) 100 ng sp6 were incubated without or with indicated inhibitors and 5 μg S10 extract for 30 min at 37°C. Remaining p6 was detected by Western blot. 10 ng of sp6BY were incubated with 5 μg S10 extract and increasing concentrations of insulin (B) or 6bK (C) for 30 min at 37°C. sp6BY was detected by measurement of fluorescence excitation. Values represent the arithmetic mean ± SD of at least three independent experiments.