Combination of mesenchymal stem cell injection with icariin for the treatment of diabetes-associated erectile dysfunction
Fig 4
Characteristics of ADSCs and effects of H2O2 and icariin on the viability of ADSCs and H2O2 treated ADSCs, respectively.
A) Flowcytometry demonstrated that most ADSCs expressed CD90, CD29 but less CD45, CD34. B) Morphology of ADSCs and multipotency of ADSCs, differentiating into adipocytes stained by Oil red O and osteocytes stained by Alizarin red S (Scale bar: 100 μm). C) Cells were treated with 0, 25, 50, 100, and 200 μM H2O2 for 12 h followed by PI staining with flow cytometry to determine cell viability, n = 7. D) Cells were treated with 0, 50, 100, 150 and 200 μM icariin under 100 μM H2O2 containing medium for 12 h incubation. Cell viability was determined using PI staining, n = 7.