Robust growth of avirulent phase II Coxiella burnetii in bone marrow-derived murine macrophages
Fig 4
NMII-infected C57BL/6J BMDM produce moderate amounts of TNF and nitric oxide.
BMDM were infected with NMII at an MOI of 50. At 8 h post-infection, cells were mock treated or treated with 100 ng/ml LPS (TLR4 ligand), 10 μg/ml zymosan (TLR2 ligand), or 10 ng/ml TNF (nitrite assay only). Following an 18 h treatment, culture supernatants were assayed for TNF by using an ELISA kit (A) and nitric oxide by determining nitrite concentration with the Griess reagent (B). Results are expressed as the means of three biological replicates from one experiment and are representative of two independent experiments. Error bars indicate the standard deviations of the means.