Ibutilide treatment protects against ER stress induced apoptosis by regulating calumenin expression in tunicamycin treated cardiomyocytes
Fig 6
Ibutilide treatment alters protein expression of ER stress markers GRP78, GRP94 and calumenin in tunicamycin treated RNC.
Protein levels GRP78 and GRP94 and calumenin were analyzed from lysates derived from RNC treated with either tunicamycin alone (model), tunicamycin and ibutilide (treatment) or untreated cardiomyocytes (control). Representative immunoblots of lysate immunoblotted with antibodies specific for (A) GRP78, (B) GRP94 or (C) Calumenin. Quantification of band densitometry is shown adjacent to the corresponding blot. All data are shown as mean ± SE (n = 3 per group). *p<0.5, **p<0.01, ***p<0.001.