Strategy of Pseudomonas pseudoalcaligenes C70 for effective degradation of phenol and salicylate
Fig 1
Genetic organization of the phe and sal operons of Pseudomonas pseudoalcaligenes C70.
(A) The genes of the phe operon—pheR, transcriptional regulator: pheKLMNOP, components of phenol hydroxylase; pheQ, ferredoxin-like gene; pheB, catechol 2,3-dioxygenase; pheC, 2-hydroxymuconic semialdehyde dehydrogenase; pheD, 2-hydroxymuconic semialdehyde hydrolase; pheE, 2-oxopent-4-dienoate hydratase; pheF, acetaldehyde dehydrogenase; pheG, 4-hydroxy-2-oxovalerate aldolase; pheH, 4-oxalocrotonate decarboxylase; pheI, 4-oxalocrotonate isomerase. (B) The genes of the sal operon—tnpA, transposase-like gene: nahW, salicylate 1-hydroxylase; nahR, regulatory gene; nahG, salicylate 1-hydroxylase; nahT, chloroplast ferredoxin-like protein coding gene; nahH, catechol 2,3-dioxygenase; nahI, hydroxymuconic semialdehyde dehydrogenase; nahN, hydroxymuconic semialdehyde hydrolase; nahL, 2-oxopent-4-enoate hydratase; nahO, acetaldehyde dehydrogenase; nahM, 2-oxo-4-hydroxypentanoate aldolase; nahK, 4-oxalocrotonate decarboxylase; nahJ, 4-oxalocrotonate isomerase; nahX, hypothetical protein coding gene; tnpISBma1, transposase-like gene.