The p53 isoform delta133p53ß regulates cancer cell apoptosis in a RhoB-dependent manner
Fig 2
Delta133p53ß controls RhoB activity in CRC cell lines.
(a) RhoB activity in HCT116, SW480 and SW620 cells was assessed by using GTPase activity assays. Results are expressed as the fold change compared with HCT116 cells and represent the mean ± SEM of four independent experiments; *, p<0.05. (b) Representative immunoblot showing levels of total and GTP-bound RhoB in HCT116, SW480 and SW620 cells. (c) RhoB activity in mock-transfected (control) and delta133p53ß-overexpressing HCT116 cells. Results are expressed as the fold change compared with control and represent the mean ± SEM of three independent experiments; *, p<0.05. (d) Representative immunoblot showing MYC-tagged delta133p53ß expression in delta133p53ß-overexpressing and mock-transfected (control) HCT116 cells. (e) RhoB activity in mock-transfected (control) SW620 cells and following transfection with two different siRNAs against delta133p53. Results are expressed as the fold change compared with control cells and represent the mean ± SEM of four independent experiments; *, p<0.05. (f) Representative immunoblot showing the levels of total and GTP-bound RhoB in control and delta133p53-depleted SW620 cells. (g) Depletion of delta133p53 isoforms in SW620 cells after siRNA transfection was assessed by RT-quantitative PCR. Results are expressed as the fold change compared with mock-transfected SW620 cells (control) and represent the mean ± SEM of four independent experiments.