The neutralizing role of IgM during early Chikungunya virus infection
Fig 1
The appearance of CHIKV-specific neutralizing antibodies is associated with a reduction of viremia.
(A) Seroneutralization was performed on sera from panel A which had known viral loads. Experiments were performed in triplicate at 1:100 dilution for detection of neutralizing IgM and IgG. The dotted lines represent the cut-off value determined from the mean—SD values from healthy control sera. (B) Neutralization titers (NT50) of total antibodies and previously-determined viral loads [26] of serum samples (panel A) collected at different times of disease onset were plotted. Each point indicates the median of RNA copies/ml targeting E1 and NT50, with the inter-quartile range. The dashed line indicates the limit of quantification (1 log10 RNA copies/reaction or 4.76 log10 RNA/ml) of the E1 positive-strand PCR assay [26].