Composition and pathogenic potential of a microbial bioremediation product used for crude oil degradation
Fig 4
A549 cytotoxicity and cytokine response.
A549 cells were exposed to dilutions in of MBP or its filtrate (1/20 dilution) either in the absence (A and B) or presence (C) of the broad-spectrum antibiotic, gentamicin. At various times, A549 cells were assessed for metabolic activity using the MTT bioreduction (A) or the Trypan Blue dye exclusion (B) assays. Supernatants from these exposures were tested for levels of pro-inflammatory cytokines and chemokines. (C) Cytokines and chemokines were found to be significantly elevated in response to some exposures. Each data point represents a representative experiment done in duplicate ± standard deviation. Asterisks indicate statistically different values compared to saline-treated controls (p<0.05).