Toll-like receptor 2 activation and serum amyloid A regulate smooth muscle cell extracellular matrix
Fig 6
TLR2 activation increases MMP9 expression and decreases tropoelastin expression.
SMCs were treated (or control-treated) with SAA, Pg LPS or LTA for 24 hours (A). Tlr2 mRNA levels are expressed relative to the control-treated sample ± SD (n = 3). SMCs were co-transfected with NFκB-luc and a Renilla construct, the latter to normalize transfection efficiency (B). Cells were treated (or control-treated) with SAA or Pg LPS for 24 hours. Data are expressed as luciferase/Renilla ± SD (n = 5). SMCs were treated with SAA, Pg LPS, LTA, Pam or FSL as in Fig 6A (C, D). Nos2 (C) and Mmp9 (D) mRNA levels are expressed relative to the control-treated sample ± SD (n = 3). SMCs were treated as in Fig 6A and Western blot analysis performed with antibodies directed against MMP9 and tubulin (E). SMCs were treated as in Fig 6D (F). Eln mRNA levels are expressed relative to the control-treated sample ± SD (n = 3). SMCs were treated as in Fig 6A and Western blot analysis performed with antibodies directed against tropoelastin and tubulin (G). SMCs were transfected as in Fig 2F (H). Cells were treated (or control-treated) with SAA, Pg LPS or LTA for 24 hours. Data are expressed as luciferase/Renilla ± SD (n = 5).